Genetic Engineering

41 MCQs

MCQs

41 questions · 35 min · 41 marks

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Sample questions

The first 5 of 41. Try each one, then tap to see the answer.

  1. Question 1

    Identify the FALSE statement.

    • ARestriction enzyme is used for the production of RFLPs.
    • BDNA ligase enzyme is used to cut DNA, creating the sticky ends.
    • CReverse transcriptase enzyme is used for making cDNA from mRNA.
    • DElectrophoresis means the separation of DNA fragments.
    Show answer

    Answer: B

    DNA ligase joins DNA fragments (molecular glue) while restriction endonucleases cut DNA to create sticky ends.

  2. Question 2

    Which method is used for obtaining the gene of interest?

    • AArtificial gene synthesis
    • BReverse transcription
    • CCleavage from chromosome
    • DAll of these
    Show answer

    Answer: D

    The gene can be synthesised directly, made from mRNA by reverse transcription or cleaved from a chromosome.

  3. Question 3

    When a gene of interest is attached to the vector, the new molecule is called:

    • ARecombinant DNA
    • BRestriction length fragment
    • CComplimentary DNA
    • DGenomic library
    Show answer

    Answer: A

    The vector carrying the gene of interest becomes recombinant DNA (rDNA).

  4. Question 4

    What is the use of plasmid in genetic engineering?

    • ATo carry DNA to a cell
    • BTo cut DNA
    • CTo join pieces of DNA
    • DTo replicate DNA
    Show answer

    Answer: A

    Plasmids act as vectors that introduce the gene of interest into host cells.

  5. Question 5

    Which of these can serve as ideal expression system for gene cloning?

    • ABacteriophages
    • BBacterial cells
    • CPlant cells
    • DAnimal cells
    Show answer

    Answer: B

    Bacterial cells are ideal because of their short generation time and simple genetic system.

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