Class 12 Biology · Chapter 8 · Test 2
PCR, GMOs and Insulin
38 MCQs
38 questions · 35 min · 38 marks
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Sample questions
The first 5 of 38. Try each one, then tap to see the answer.
Question 1
Which bacterium is used in the production of insulin by genetic engineering?
- ASaccharomyces
- BRhizobium
- CEscherichia
- DMycobacterium
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Answer: C
Escherichia coli is used as the host.
Question 2
Which technique is used for cloning of gene/DNA?
- ARFLP analysis
- BPolymerase Chain Reaction (PCR)
- CElectroporation
- DGel electrophoresis
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Answer: B
PCR makes many identical copies of a DNA fragment.
Question 3
In PCR, the optimum temperature for carrying out the extension of primer is:
- A98°C
- B72°C
- C60°C
- D37°C
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Answer: B
Taq polymerase works best at 72°C and this step takes about one minute.
Question 4
Which enzyme is used for the extension of primer in PCR?
- ADNA polymerase
- BTranscriptase
- CDNA ligase
- DRestriction endonuclease
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Answer: A
Taq DNA polymerase adds nucleotides to the 3' end of the primer.
Question 5
What is true for a Genetically Modified Organism?
- AGenes have been introduced
- BGenes have been enhanced
- CGenes have been deleted
- DAll of these
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Answer: D
A GMO is an organism whose genome has been changed in any of these ways.
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